Publications



  1. Abdelkrim, Y.Z., Banroques, J, & Tanner, N.K.* (2021) Known inhibitors of RNA helicases and their therapeutic potential. Methods Mol Biol pp.35-52, 2021, 10.1007/978-1-0716-0935-4_3
  2. Yeter-Alat, H., Belgareh-Touzé, N., Huvelle, E., Mokdadi, M., Banroques, J., and Tanner, N.K.* (2020) The DEAD-box RNA helicase Ded1 is a signal-recognition-particle-associated factor that is regulated by SRP21. https://www.biorxiv.org/content/10.1101/2020.11.08.373522v1
  3. Lacoux, C., Wacheul, L., Saraf, K., Pythoud, N., Huvelle, E., Figaro, S., Graille, M., Carapito, C., Lafontaine, D.L.J & Heurgué-Hamard, V. (2020) The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis Nucleic Acids Res. 48; 12310-12325. doi: 10.1093/nar/gkaa972
  4. Roychowdhury, A., Joret, C., Bourgeois, G., Heurgué-Hamard, V., Lafontaine, D.L.J & Graille, M. (2019) The DEAH RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis. Nucleic Acids Res. 47; 7548-7563. doi: 10.1093/nar/gkz529
  5. Raj, S., Bagchi, D., Valle Orero, J., Banroques, B., Tanner, N.K., Croquette, V. (2019) Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers. Nucleic Acids Res. 47; 3699-3710.
  6. Geffroy L, Bizebard T, Aoyama R, Ueda T, Bockelmann U. (2019) Force measurements show that uL4 and uL24 mechanically stabilize a fragment of 23S rRNA essential for ribosome assembly. RNA 25:472-480. doi: 10.1261/rna.067504.118.
  7. Bizebard T, Arluison V, Bockelmann U. (2018) Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing. Methods Mol Biol. 1737:301-319. doi: 10.1007/978-1-4939-7634-8_17.
  8. Abdelkrim, Y.Z., Harigua-Souiai, E., Barhoumi, M., Banroques, J., Blondel, A., Guizani, I., Tanner, N.K. (2018) The steroid derivative 6-aminocholestanol inhibits the DEAD-box helicase eIF4A (LieIF4A) from the Trypanosomatid parasite Leishmania by perturbing the RNA and ATP binding sites. Mol Biochem Parasit. 226: 9-19. https://doi.org/10.1016/j.molbiopara.2018.10.001.
  9. Geffroy L, Mangeol P, Bizebard T, Bockelmann U. (2018) RNA Unzipping and Force Measurements with a Dual Optical Trap. Methods Mol Biol. 1665:25-41. doi: 10.1007/978-1-4939-7271-5_2.
  10. Harigua-Souiai, E., Abdelkrim, Y.Z., Bassoumi-Jamoussi, I., Zakraoui, O., Bouvier, G., Essafi-Benkhadir, K., Banroques, J., Desdouits, N., Munier-Lehmann, H., Barhoumi, M., Tanner, N.K., Nilges, M., Blondel, A., Guizani, I. (2017) Identification of Novel Leishmanicidal Molecules by Virtual Screening Targeting Leishmania Eukaryotic Translation Initiation Factor 4A. PLoS NTD, https://doi.org/10.1371/journal.pntd.000616.
  11. Zorbas, C.,  Nicolas, E., Wacheul, L., Huvelle, E., Heurgué-Hamard, V & Lafontaine D.L.J.(2015) The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol. Biol. Cell, 26, 2080-2095
  12. Indrisiunaite G, Pavlov MY, Heurgué-Hamard V, Ehrenberg M. (2015) On the pH Dependence of Class-1 RF-Dependent Termination of mRNA Translation. J. Mol. Biol. 427, 1848-60.
  13. Bizebard, T., & Dreyfus, M. (2015) A FRET-based, continuous assay for the helicase activity of DEAD-box proteins. Methods Mol Biol 1259; 199-209 doi: 10.1007/978-1-4939-2214-7_13.
  14. Létoquart, J.*,Huvelle, E.*, Wacheul, L.*, Bourgeois, G., Zorbas, C., Graille, M.#, Heurgué-Hamard, V.#, & Lafontaine D.L.J.# (2014) Structural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes. Proc Natl Acad Sci U S A. 111(51):E5518-26 * Authors contributed equally. / # Corresponding authors
  15. Banroques J, Tanner NK. (2015) Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box RNA Helicases. Methods Mol Biol;1259:165-81. doi: 10.1007/978-1-4939-2214-7_11.
  16. Cordin, O, Hahn, D, Alexander, R., Gautam, A., Saveanu, C., Barrass, J.D., Beggs, J.D. (2014) Brr2p carboxy-terminal Sec63 domain modulates Prp16 splicing RNA helicase Nucleic Acids Res. 42:13897-910. doi: 10.1093/nar/gku1238.
  17. Mota S, Vieira N, Barbosa S, Delaveau T, Torchet C, Le Saux A, Garcia M, Pereira A, Lemoine S, Coulpier F, Darzacq X, Benard L, Casal M, Devaux F, Paiva S. (2014) Role of the DHH1 Gene in the Regulation of Monocarboxylic Acids Transporters Expression in Saccharomyces cerevisiae. PLoS One. 9(11):e111589. doi: 10.1371/journal.pone.0111589.
  18. Senissar, M., Saux, A. L., Belgareh-Touzé, N., Adam, C., Banroques, J., & Tanner, N. K. (2014). The DEAD-box helicase Ded1 from yeast is an mRNP cap-associated protein that shuttles between the cytoplasm and nucleus. Nucleic Acids Res. 42:10005-22. doi: 10.1093/nar/gku584.
  19. Roque, S., Cerciat, M., Gaugué, I., Mora, L., Floch, A., de Zamaroczy, M., Heurgué-Hamard, V., & Kervestin, S. (2014) Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae. RNA 21, 124-134 2012
  20. Bercy, M., Mangeol, P., Bizebard, T., Tanner, N.K., Banroques J., & Bockelmann, U. (2013) "Investigating RNA structure and folding with optical tweezers," in Structure and Folding of RNA, C. Hammann & D. Klostermeier, eds, Gruyter, USA. pp 157-179.
  21. Barhoumi, M., Meddeb-Garnaoui, A., Tanner, N.K., Banroques, J., Kaabi, B., and Guizani1, I. (2013) DEAD-box proteins, like Leishmania eIF4A, modulate interleukin (IL)-12, IL-10 and tumor necrosis factor-alpha production by human monocytes. Parasite Immunol. 35, 194-199.
  22. Iost, I., Bizebard, T., & Dreyfus, M. (2013) Functions of DEAD-box proteins in bacteria: Current knowledge and pending questions. Biochem. Biophys. Acta 1829, 866-877.
  23. Figaro, S., Wacheul, L., Schillewaert, S., Graille, M., Huvelle, E., Mongeard, R., Zorbas, C., Lafontaine, D. & Heurgué-Hamard, V. (2012) Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575. Mol Cell Biol, 32, 2254-2267.
  24. Graille, M., Figaro, S., Kervestin, S., Buckingham, R. H., Liger, D. & Heurgué-Hamard, V. (2012) Methylation of class I translation termination factors: Structural and functional aspects, Biochimie, 94, 1533-1543.
  25. Barhoumi, M., Garnaoui, A., Kaabi, B., Tanner, N.K., and Guizani, I. (2011) Leishmania infantum LeIF and its recombinant polypeptides modulate interleukin IL-12p70, IL-10 and tumour necrosis factor-α production by human monocytes. Parasite Immunol. 33, 583–588.
  26. Liger, D*., Mora, L*., Lazar, N., Figaro, S., Henri, J., Scrima, N., Buckingham R. H., van Tilbeurgh, H., Heurgué-Hamard, V.** & Graille, M **(2011) Mechanism of activation of methyltransferases involved in translation by the Trm112 "hub" protein.  Nucleic Acids Res. 39, 6249-6259. * Co-first authors **Co-corresponding authors
  27. Dreyfus, M. & Heurgué-Hamard, V. (2011) Termination troubles in Escherichia coli K12. Mol. Microbiol. 79, 288-291.
  28. Proux, F., Dreyfus, M., Iost, II (2011) Identification of the sites of action of SrmB, a DEAD-box RNA helicase involved in Escherichia coli ribosome assembly. Mol Microbiol, 82, 300-311.
  29. Mangeol, P., Bizebard, T., Chiaruttini, C., Dreyfus, M., Springer, M., & Bockelmann, U. (2011) Probing ribosomal protein–RNA interactions with an external force. Proc. Natl. Acad. Sci. U.S.A. 108, 18272-18276
  30. Banroques, J., Cordin, O., Doere, M., Linder, P., & Tanner, N. K. (2011) Analyses of the functional regions of DEAD-Box RNA "Helicases" with deletion and chimera constructs tested in vivo and in vitro. J Mol Biol, 413, 451-472.
  31. Cartier, G., Lorieux, F., Allemand, F., Dreyfus, M., and Bizebard, T. (2010) Cold adaptation in DEAD-box proteins Biochemistry 49, 2636-2646.
  32. Banroques, J., Doere, M., Dreyfus, M., Linder, P., and Tanner, N. K. (2010) Motif III in superfamily 2 "helicases" helps convert the binding energy of ATP into a high-affinity RNA binding site in the yeast DEAD-box protein Ded1 J Mol Biol 396, 949-966.
  33. Trubetskoy, D., Proux, F., Allemand, F., Dreyfus, M. and Iost, I. (2009) SrmB, a DEAD-box helicase involved in Escherichia coli ribosome assembly, is specifically targeted to 23S rRNA in vivo. Nucleic Acids Res. 37, 6540-6549.
  34. Dreyfus, M. (2009) Killer and protective ribosomes. Prog Nucleic Acid Res Mol Biol, 85, 423-466.
  35. Figaro, S., Scrima, N., Buckingham, R.H. & Heurgué-Hamard, V. (2008) HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1. FEBS Lett. 582, 2352-2356.
  36. Banroques, J., Cordin, O., Doère, M., Linder, P., Tanner, N.K. (2008) A conserved phenylalanine of motif IV in superfamily 2 helicases is required for cooperative, ATP-dependent, binding of RNA substrates in DEAD-box proteins. Molecular and Cellular Biology 28, 3359-3371.
  37. Mora, L., Heurgue-Hamard, V., de Zamaroczy, M., Kervestin, S. and Buckingham, R. H. (2007) Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo. J. Biol. Chem. 282, 35638-35645.
  38. Cordin, O., Banroques, J., Tanner, N.K., Linder, P. (2006) The DEAD-box protein family of RNA helicases. Gene 367, 17-37.
  39. Heurgué-Hamard, V., Graille, M., Scrima, N., Ulryck, N., Champ, S., van Tilbeurgh, H. and Buckingham, R. H. (2006) The zinc-finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast. J. Biol. Chem. 281, 36140-36148.
  40. Graille, M., Heurgué-Hamard, V., Champ, S., Mora, L., Scrima, N., Ulryck, N., van Tilbeurgh, H. & Buckingham, R.H. (2005) Molecular basis for bacterial class I release factor methylation by PrmC. Mol. Cell, 20, 917-927.
  41. Heurgue-Hamard, V., Champ, S., Mora, L., Merkulova-Rainon, T., Kisselev, L. L., and Buckingham, R. H. (2005). The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene. J. Biol. Chem. 280, 2439-2445.
  42. Guillerez, J., Lopez, P.J., Proux, F., Launay, H. and Dreyfus, M. (2005) A mutation in T7 RNA polymerase that facilitates promoter clearance. Proc. Natl. Acad. Sci. USA, 102, 5958-5963.
  43. Bizebard, T., Ferlenghi, I., Iost, I. and Dreyfus, M. (2004) Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases. Biochemistry, 43, 7857-7866.
  44. Cordin, O, Tanner, N.K., Doère, M., Linder, P., Banroques, J. (2004) The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity. EMBO Journal 23, 2478-2487.
  45. Tanner, N.K., Cordin, O., Banroques, J., Doère, M., and Linder, P. (2003) The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis. Molecular Cell 11, 127-138.
  46. Mora, L., Heurgué-Hamard, V., Champ, S., Ehrenberg, M., Kisselev, L. & Buckingham, R.H. (2003) The essential role of the invariant GGQ motif in the function and the stability in vivo of bacterial release factors RF1 and RF2. Mol. Microbiol. 47, 267-275.
  47. Heurgué-Hamard, V., Champ, S., Engstöm, Å., Ehrenberg, M. & Buckingham, R.H. (2002) The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors. EMBO J. 21, 769-778.
  48. Dreyfus, M. and Régnier, P. (2002) The poly(A) tail of mRNAs: bodyguard in eucaryotes, scavenger in bacteria. Cell, 111, 611-613.
  49. Tanner, N.K. and Linder, P. (2001) DExD/H-box helicases: from generic motors to specific dissociation functions. Molecular Cell 8, 251-262.
  50. Heurgué-Hamard, V., Dincbas, V., Buckingham, R.H. & Ehrenberg, M. (2000) Origins of minigene-dependent growth inhibition in bacterial cells. EMBO J. 19, 2701-9.
  51. Dinçbas-Renqvist, V., Engström, Å., Mora, L., Heurgué-Hamard, V., Buckingham, R.H. & Ehrenberg, M. (2000) A post-translational modification in the GGQ motif of RF2 from Escherichia coli stimulates termination of translation. EMBO J. 19, 6900-6907.